› 1ou6

X-ray diffraction
2.07Å resolution

Biosynthetic thiolase from Zoogloea ramigera in complex with acetyl-O-pantetheine-11-pivalate

Released:
DOI: 10.2210/pdb1ou6/pdb
PDB entry 1ou6 coloured by chain, front view.
PDB entry 1ou6 coloured by chain, side view.
PDB entry 1ou6 coloured by chain, top view.
Source organism: Zoogloea ramigera
Entry authors: Kursula P, Schmitz W, Wierenga RK

Function and Biology Details

Reaction catalysed: 
(1a) acetyl-CoA + [acetyl-CoA C-acetyltransferase]-L-cysteine = [acetyl-CoA C-acetyltransferase]-S-acetyl-L-cysteine + CoA
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139369 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Acetyl-CoA acetyltransferase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 392 amino acids
Theoretical weight: 40.56 KDa
Source organism: Zoogloea ramigera
Expression system: Escherichia coli
UniProt:
  • Canonical: P07097 (Residues: 2-11, 12-392; Coverage: 100%)
Gene names: phaA, phbA
Sequence domains:
Structure domains: Peroxisomal Thiolase; Chain A, domain 1

Ligands and Environments

2 bound ligands:
Ligand SO4 4 x SO4
Ligand 168 2 x 168
1 modified residue:
Ligand CSO 4 x CSO

Experiments and Validation Details

Entry percentile scores
X-ray source: ENRAF-NONIUS FR591
Spacegroup: P21
Unit cell:
a: 84.38Å b: 78.95Å c: 148.82Å
α: 90° β: 92.96° γ: 90°
R-values:
R R work R free
0.163 0.161 0.212
Expression system: Escherichia coli

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