› 1ox4

X-ray diffraction
2.5Å resolution

TOWARDS UNDERSTANDING THE MECHANISM OF THE COMPLEX CYCLIZATION REACTION CATALYZED BY IMIDAZOLE GLYCEROPHOSPHATE SYNTHASE

Released:
DOI: 10.2210/pdb1ox4/pdb
PDB entry 1ox4 coloured by chain, front view.
PDB entry 1ox4 coloured by chain, side view.
PDB entry 1ox4 coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Toward understanding the mechanism of the complex cyclization reaction catalyzed by imidazole glycerolphosphate synthase: crystal structures of a ternary complex and the free enzyme.
Chaudhuri BN, Lange SC, Myers RS, Davisson VJ, Smith JL
Biochemistry 42 7003-12 (2003)
PMID: 12795595

Function and Biology Details

Reactions catalysed: 
L-glutamine + H(2)O = L-glutamate + NH(3)
(1a) L-glutamine + H(2)O = L-glutamate + NH(3)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152640 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Imidazole glycerol phosphate synthase hisHF Chain: A
Molecule details ›
Chain: A
Length: 555 amino acids
Theoretical weight: 61.42 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P33734 (Residues: 1-552; Coverage: 100%)
Gene names: HIS7, YBR1640, YBR248C
Sequence domains:
Structure domains:
Imidazole glycerol phosphate synthase hisHF Chain: B
Molecule details ›
Chain: B
Length: 555 amino acids
Theoretical weight: 61.57 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P33734 (Residues: 1-552; Coverage: 100%)
Gene names: HIS7, YBR1640, YBR248C
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand SO4 4 x SO4
Ligand NI 2 x NI
Ligand POP 2 x POP
1 modified residue:
Ligand CYD 1 x CYD

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P212121
Unit cell:
a: 98.5Å b: 112Å c: 115.3Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.228 0.228 0.26
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Gates of enzymes.
Gora et al. (2013)
5 more

7 mentions without citation

SCOPPI: a structural classification of protein-protein interfaces.
Winter et al. (2006)
6 more