› 1ox6

X-ray diffraction
2.4Å resolution

TOWARDS UNDERSTANDING THE MECHANISM OF THE COMPLEX CYCLIZATION REACTION CATALYZED BY IMIDAZOLE GLYCEROPHOSPHATE SYNTHASE

Released:
DOI: 10.2210/pdb1ox6/pdb
PDB entry 1ox6 coloured by chain, front view.
PDB entry 1ox6 coloured by chain, side view.
PDB entry 1ox6 coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Toward understanding the mechanism of the complex cyclization reaction catalyzed by imidazole glycerolphosphate synthase: crystal structures of a ternary complex and the free enzyme.
Chaudhuri BN, Lange SC, Myers RS, Davisson VJ, Smith JL
Biochemistry 42 7003-12 (2003)
PMID: 12795595

Function and Biology Details

Reactions catalysed: 
L-glutamine + H(2)O = L-glutamate + NH(3)
(1a) L-glutamine + H(2)O = L-glutamate + NH(3)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152640 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Imidazole glycerol phosphate synthase hisHF Chains: A, B
Molecule details ›
Chains: A, B
Length: 555 amino acids
Theoretical weight: 61.42 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P33734 (Residues: 1-552; Coverage: 100%)
Gene names: HIS7, YBR1640, YBR248C
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand NI 2 x NI
Ligand SO4 5 x SO4
Ligand POP 1 x POP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P212121
Unit cell:
a: 97Å b: 112Å c: 114.8Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.228 0.228 0.255
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Gates of enzymes.
Gora et al. (2013)
5 more

6 mentions without citation

The Role of Gene Elongation in the Evolution of Histidine Biosynthetic Genes.
Del Duca et al. (2020)
5 more