› 1oxe

X-ray diffraction
1.15Å resolution

Expansion of the Genetic Code Enables Design of a Novel "Gold" Class of Green Fluorescent Proteins

Released:
DOI: 10.2210/pdb1oxe/pdb
PDB entry 1oxe coloured by chain, front view.
PDB entry 1oxe coloured by chain, side view.
PDB entry 1oxe coloured by chain, top view.
Source organism: cfp marker plasmid pWM1009
Primary publication:
Expansion of the genetic code enables design of a novel "gold" class of green fluorescent proteins.
Bae JH, Rubini M, Jung G, Wiegand G, Seifert MH, Azim MK, Kim JS, Zumbusch A, Holak TA, Moroder L, Huber R, Budisa N
J Mol Biol 328 1071-81 (2003)
PMID: 12729742

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-162365 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
cyan fluorescent protein cfp Chain: A
Molecule details ›
Chain: A
Length: 227 amino acids
Theoretical weight: 25.8 KDa
Source organism: cfp marker plasmid pWM1009
Expression system: Escherichia coli
Structure domains: Green fluorescent protein

Ligands and Environments

No bound ligands
1 modified residue:
Ligand CRF 1 x CRF

Experiments and Validation Details

Entry percentile scores
X-ray source: MPG/DESY, HAMBURG BEAMLINE BW6
Spacegroup: P212121
Unit cell:
a: 50.952Å b: 62.768Å c: 69.519Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.237 0.223 0.236
Expression system: Escherichia coli

Quick links

Citations

26 review citations

Adding new chemistries to the genetic code.
Liu et al. (2010)
25 more

2 mentions without citation

CLICK--topology-independent comparison of biomolecular 3D structures.
Nguyen et al. (2011)
1 more