› 1oyi

Solution NMR

Solution structure of the Z-DNA binding domain of the vaccinia virus gene E3L

Released:
DOI: 10.2210/pdb1oyi/pdb
PDB entry 1oyi coloured by chain, ensemble of 20 models, front view.
PDB entry 1oyi coloured by chain, ensemble of 20 models, side view.
PDB entry 1oyi coloured by chain, ensemble of 20 models, top view.
Source organism: Vaccinia virus
Primary publication:
The solution structure of the N-terminal domain of E3L shows a tyrosine conformation that may explain its reduced affinity to Z-DNA in vitro.
Kahmann JD, Wecking DA, Putter V, Lowenhaupt K, Kim YG, Schmieder P, Oschkinat H, Rich A, Schade M
Proc Natl Acad Sci U S A 101 2712-7 (2004)
PMID: 14981270

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-183102 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Double-stranded RNA-binding protein Chain: A
Molecule details ›
Chain: A
Length: 82 amino acids
Theoretical weight: 9.19 KDa
Source organism: Vaccinia virus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q86638 (Residues: 2-78; Coverage: 41%)
Gene name: E3L
Sequence domains: Adenosine deaminase z-alpha domain
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: torsion angle dynamics
Expression system: Escherichia coli BL21

Quick links

Citations

3 review citations

From keys to bulldozers: expanding roles for winged helix domains in nucleic-acid-binding proteins.
Harami et al. (2013)
2 more

7 mentions without citation

Poxvirus proteomics and virus-host protein interactions.
Van Vliet et al. (2009)
6 more