› 1oys

X-ray diffraction
2.4Å resolution

Crystal Structure of the Phosphorolytic Exoribonuclease RNase PH from Bacillus subtilis

Released:
DOI: 10.2210/pdb1oys/pdb
Model geometry
Fit model/data
PDB entry 1oys coloured by chain, front view.
PDB entry 1oys coloured by chain, side view.
PDB entry 1oys coloured by chain, top view.
Source organism: Bacillus subtilis
Primary publication:
Crystal structure of the phosphorolytic exoribonuclease RNase PH from Bacillus subtilis and implications for its quaternary structure and tRNA binding.
Harlow LS, Kadziola A, Jensen KF, Larsen S
Protein Sci 13 668-77 (2004)
PMID: 14767080

Function and Biology Details

Reaction catalysed: 
tRNA(n+1) + phosphate = tRNA(n) + a nucleoside diphosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151250 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribonuclease PH Chain: A
Molecule details ›
Chain: A
Length: 245 amino acids
Theoretical weight: 26.63 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli
UniProt:
  • Canonical: P28619 (Residues: 1-245; Coverage: 100%)
Gene names: BSU28370, rph
Sequence domains:
Structure domains: GHMP Kinase, N-terminal domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
Spacegroup: P41212
Unit cell:
a: 63.23Å b: 63.23Å c: 203Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.258 0.258 0.284
Expression system: Escherichia coli

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Citations

9 review citations

Messenger RNA turnover in eukaryotes: pathways and enzymes.
Meyer et al. (2004)
8 more

1 mention without citation

Crystal structure of the phosphorolytic exoribonuclease RNase PH from Bacillus subtilis and implications for its quaternary structure and tRNA binding.
Harlow et al. (2004)