› 1p0e

X-ray diffraction
2.4Å resolution

CRYSTAL STRUCTURE OF ZYMOMONAS MOBILIS tRNA-GUANINE TRANSGLYCOSYLASE (TGT) COCRYSTALLISED WITH PREQ1 AT PH 5.5

Released:
DOI: 10.2210/pdb1p0e/pdb
PDB entry 1p0e coloured by chain, front view.
PDB entry 1p0e coloured by chain, side view.
PDB entry 1p0e coloured by chain, top view.
Source organism: Zymomonas mobilis
Primary publication:
Flexible adaptations in the structure of the tRNA-modifying enzyme tRNA-guanine transglycosylase and their implications for substrate selectivity, reaction mechanism and structure-based drug design.
Brenk R, Stubbs MT, Heine A, Reuter K, Klebe G
Chembiochem 4 1066-77 (2003)
PMID: 14523925

Function and Biology Details

Reaction catalysed: 
Guanine(34) in tRNA + 7-aminomethyl-7-carbaguanine = 7-aminomethyl-7-carbaguanine(34) in tRNA + guanine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151274 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Queuine tRNA-ribosyltransferase Chain: A
Molecule details ›
Chain: A
Length: 386 amino acids
Theoretical weight: 42.93 KDa
Source organism: Zymomonas mobilis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P28720 (Residues: 2-386; Coverage: 100%)
Gene names: ZMO0363, tgt
Sequence domains: Queuine tRNA-ribosyltransferase
Structure domains: Queuine tRNA-ribosyltransferase-like

Ligands and Environments

2 bound ligands:
Ligand ZN 1 x ZN
Ligand PRF 1 x PRF
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: C2221
Unit cell:
a: 64.47Å b: 91.87Å c: 165.83Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.206 0.206 0.252
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Molecular recognition in chemical and biological systems.
Persch et al. (2015)
3 more

5 mentions without citation

Base ionization and ligand binding: how small ribozymes and riboswitches gain a foothold in a protein world.
Liberman et al. (2011)
4 more