› 1p33

X-ray diffraction
2.86Å resolution

Pteridine reductase from Leishmania tarentolae complex with NADPH and MTX

Released:
DOI: 10.2210/pdb1p33/pdb
PDB entry 1p33 coloured by chain, front view.
PDB entry 1p33 coloured by chain, side view.
PDB entry 1p33 coloured by chain, top view.
Source organism: Leishmania tarentolae
Primary publication:
Structure of pteridine reductase (PTR1) from Leishmania tarentolae.
Zhao H, Bray T, Ouellette M, Zhao M, Ferre RA, Matthews D, Whiteley JM, Varughese KI
Acta Crystallogr D Biol Crystallogr 59 1539-44 (2003)
PMID: 12925782

Function and Biology Details

Reaction catalysed: 
5,6,7,8-tetrahydrobiopterin + 2 NADP(+) = biopterin + 2 NADPH
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154662 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pteridine reductase 1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 289 amino acids
Theoretical weight: 30.77 KDa
Source organism: Leishmania tarentolae
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P42556 (Residues: 1-289; Coverage: 100%)
Gene names: LTDH, PTR1
Sequence domains: Enoyl-(Acyl carrier protein) reductase
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments


Cofactor: Ligand NDP 4 x NDP
1 bound ligand:
Ligand MTX 4 x MTX
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P212121
Unit cell:
a: 91.301Å b: 96.103Å c: 195.545Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.213 0.213 0.251
Expression system: Escherichia coli BL21

Quick links

Citations

1 review citation

Medium- and short-chain dehydrogenase/reductase gene and protein families : the SDR superfamily: functional and structural diversity within a family of metabolic and regulatory enzymes.
Kavanagh et al. (2008)
9 other articles

39 mentions without citation

The protective role of pregnancy in breast cancer.
Russo et al. (2005)
38 more