› 1p4a

X-ray diffraction
2.22Å resolution

Crystal Structure of the PurR complexed with cPRPP

Released:
DOI: 10.2210/pdb1p4a/pdb
PDB entry 1p4a coloured by chain, front view.
PDB entry 1p4a coloured by chain, side view.
PDB entry 1p4a coloured by chain, top view.
Source organism: Bacillus subtilis
Primary publication:
Functional dissection of the Bacillus subtilis pur operator site.
Bera AK, Zhu J, Zalkin H, Smith JL
J Bacteriol 185 4099-109 (2003)
PMID: 12837784

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153426 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pur operon repressor Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 285 amino acids
Theoretical weight: 31.26 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli
UniProt:
  • Canonical: P37551 (Residues: 1-285; Coverage: 100%)
Gene names: BSU00470, purR, yabI
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand PCP 4 x PCP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-ID-B
Spacegroup: P21
Unit cell:
a: 58.371Å b: 135.718Å c: 82.071Å
α: 90° β: 95.1° γ: 90°
R-values:
R R work R free
0.18 0.177 0.234
Expression system: Escherichia coli

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Citations

5 review citations

Nucleotide metabolism and its control in lactic acid bacteria.
Kilstrup et al. (2005)
4 more

4 mentions without citation

Crystal structure of Bacillus anthracis virulence regulator AtxA and effects of phosphorylated histidines on multimerization and activity.
Hammerstrom et al. (2015)
3 more