› 1p6d

X-ray diffraction
2Å resolution

STRUCTURE OF THE D55N MUTANT OF PHOSPHOLIPASE C FROM BACILLUS CEREUS IN COMPLEX WITH (3S)-3,4,DI-N-HEXANOYLOXYBUTYL-1-PHOSPHOCHOLINE

Released:
DOI: 10.2210/pdb1p6d/pdb
PDB entry 1p6d coloured by chain, front view.
PDB entry 1p6d coloured by chain, side view.
PDB entry 1p6d coloured by chain, top view.
Source organism: Bacillus cereus
Primary publication:
Using X-ray crystallography of the Asp55Asn mutant of the phosphatidylcholine-preferring phospholipase C from Bacillus cereus to support the mechanistic role of Asp55 as the general base.
Antikainen NM, Monzingo AF, Franklin CL, Robertus JD, Martin SF
Arch Biochem Biophys 417 81-6 (2003)
PMID: 12921783

Function and Biology Details

Reaction catalysed: 
A phosphatidylcholine + H(2)O = 1,2-diacyl-sn-glycerol + phosphocholine
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140704 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phospholipase C Chain: A
Molecule details ›
Chain: A
Length: 245 amino acids
Theoretical weight: 28.42 KDa
Source organism: Bacillus cereus
Expression system: Escherichia coli
UniProt:
  • Canonical: P09598 (Residues: 39-283; Coverage: 95%)
Gene name: plc
Sequence domains: Zinc dependent phospholipase C
Structure domains: P1 Nuclease

Ligands and Environments

2 bound ligands:
Ligand ZN 3 x ZN
Ligand 3PC 1 x 3PC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P43212
Unit cell:
a: 89.933Å b: 89.933Å c: 73.936Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.177 0.174 0.203
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Recent research progress with phospholipase C from Bacillus cereus.
Lyu et al. (2016)
4 other articles

3 mentions without citation

Bacterial Sphingomyelinases and Phospholipases as Virulence Factors.
Flores-Díaz et al. (2016)
2 more