› 1p6o

X-ray diffraction
1.14Å resolution

The crystal structure of yeast cytosine deaminase bound to 4(R)-hydroxyl-3,4-dihydropyrimidine at 1.14 angstroms.

Released:
DOI: 10.2210/pdb1p6o/pdb
PDB entry 1p6o coloured by chain, front view.
PDB entry 1p6o coloured by chain, side view.
PDB entry 1p6o coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
The 1.14 A crystal structure of yeast cytosine deaminase: evolution of nucleotide salvage enzymes and implications for genetic chemotherapy.
Ireton GC, Black ME, Stoddard BL
Structure 11 961-72 (2003)
PMID: 12906827

Function and Biology Details

Reaction catalysed: 
Cytosine + H(2)O = uracil + NH(3)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171310 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cytosine deaminase Chains: A, B
Molecule details ›
Chains: A, B
Length: 161 amino acids
Theoretical weight: 17.76 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: Q12178 (Residues: 1-158; Coverage: 100%)
Gene names: FCY1, YP9499.17, YPR062W
Sequence domains: Cytidine and deoxycytidylate deaminase zinc-binding region
Structure domains: Cytidine Deaminase, domain 2

Ligands and Environments

4 bound ligands:
Ligand ZN 2 x ZN
Ligand HPY 2 x HPY
Ligand ACY 3 x ACY
Ligand CA 2 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P212121
Unit cell:
a: 53.88Å b: 70.417Å c: 71.523Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.112 0.11 0.152
Expression system: Escherichia coli

Quick links

Citations

11 review citations

The APOBEC3 family of retroelement restriction factors.
Refsland et al. (2013)
10 more

16 mentions without citation

Structural biology of the purine biosynthetic pathway.
Zhang et al. (2008)
15 more