› 1p7d

X-ray diffraction
2.95Å resolution

Crystal structure of the Lambda Integrase (residues 75-356) bound to DNA

Released:
DOI: 10.2210/pdb1p7d/pdb
PDB entry 1p7d coloured by chain, front view.
PDB entry 1p7d coloured by chain, side view.
PDB entry 1p7d coloured by chain, top view.
Source organism: Lambdavirus lambda
Primary publication:
A conformational switch controls the DNA cleavage activity of lambda integrase.
Aihara H, Kwon HJ, Nunes-Düby SE, Landy A, Ellenberger T
Mol Cell 12 187-98 (2003)
PMID: 12887904

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-114364 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Integrase Chains: A, B
Molecule details ›
Chains: A, B
Length: 283 amino acids
Theoretical weight: 31.91 KDa
Source organism: Escherichia virus Lambda
Expression system: Escherichia coli
UniProt:
  • Canonical: P03700 (Residues: 74-356; Coverage: 80%)
Gene name: int
Sequence domains:
Structure domains:
5'-D(*CP*AP*AP*TP*GP*CP*CP*AP*AP*CP*TP*TP*T)-3' Chains: C, E
Molecule details ›
Chains: C, E
Length: 13 nucleotides
Theoretical weight: 3.91 KDa
Source organism: Lambdavirus lambda
Expression system: Not provided
26-MER Chains: D, F
Molecule details ›
Chains: D, F
Length: 26 nucleotides
Theoretical weight: 8.08 KDa
Source organism: Lambdavirus lambda
Expression system: Not provided

Ligands and Environments

No bound ligands
1 modified residue:
Ligand PTR 2 x PTR

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P21
Unit cell:
a: 59.021Å b: 106.161Å c: 73.165Å
α: 90° β: 92.1° γ: 90°
R-values:
R R work R free
0.231 0.231 0.259
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

3 review citations

Mechanisms of site-specific recombination.
Grindley et al. (2006)
2 more

7 mentions without citation

The protein interaction map of bacteriophage lambda.
Rajagopala et al. (2011)
6 more