› 1p7j

X-ray diffraction
2.1Å resolution

Crystal structure of engrailed homeodomain mutant K52E

Released:
DOI: 10.2210/pdb1p7j/pdb
PDB entry 1p7j coloured by chain, front view.
PDB entry 1p7j coloured by chain, side view.
PDB entry 1p7j coloured by chain, top view.
Source organism: Drosophila melanogaster
Primary publication:
Crystal structures of engrailed homeodomain mutants: implications for stability and dynamics.
Stollar EJ, Mayor U, Lovell SC, Federici L, Freund SM, Fersht AR, Luisi BF
J Biol Chem 278 43699-708 (2003)
PMID: 12923178

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136264 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Segmentation polarity homeobox protein engrailed Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 59 amino acids
Theoretical weight: 7.22 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli
UniProt:
  • Canonical: P02836 (Residues: 454-512; Coverage: 11%)
Gene names: CG9015, en
Sequence domains: Homeodomain
Structure domains: Homeodomain-like

Ligands and Environments

1 bound ligand:
Ligand NHE 1 x NHE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX14.2
Spacegroup: P212121
Unit cell:
a: 44.85Å b: 51.679Å c: 112.785Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.2 0.198 0.241
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Lone pair-π interactions in biological systems: occurrence, function, and physical origin.
Kozelka J. (2017)
1 more

3 mentions without citation

The helix-turn-helix motif as an ultrafast independently folding domain: the pathway of folding of Engrailed homeodomain.
Religa et al. (2007)
2 more