› 1p99

X-ray diffraction
1.7Å resolution

1.7A crystal structure of protein PG110 from Staphylococcus aureus

Released:
DOI: 10.2210/pdb1p99/pdb
PDB entry 1p99 coloured by chain, front view.
PDB entry 1p99 coloured by chain, side view.
PDB entry 1p99 coloured by chain, top view.
Source organism: Staphylococcus aureus subsp. aureus Mu50
Primary publication:
The membrane-associated lipoprotein-9 GmpC from Staphylococcus aureus binds the dipeptide GlyMet via side chain interactions.
Williams WA, Zhang RG, Zhou M, Joachimiak G, Gornicki P, Missiakas D, Joachimiak A
Biochemistry 43 16193-202 (2004)
PMID: 15610013

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-101966 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Lipoprotein Chain: A
Molecule details ›
Chain: A
Length: 295 amino acids
Theoretical weight: 32.51 KDa
Source organism: Staphylococcus aureus subsp. aureus Mu50
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0H3JTG5 (Residues: 19-280; Coverage: 100%)
Gene name: SAV0464
Sequence domains: NlpA lipoprotein
Structure domains: Periplasmic binding protein-like II

Ligands and Environments

2 bound ligands:
Ligand GLY 1 x GLY
Ligand MET 1 x MET
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P212121
Unit cell:
a: 40.496Å b: 73.883Å c: 92.246Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.2 0.2 0.236
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

High-throughput crystallography for structural genomics.
Joachimiak A. (2009)
2 more

5 mentions without citation

The membrane-associated lipoprotein-9 GmpC from Staphylococcus aureus binds the dipeptide GlyMet via side chain interactions.
Williams et al. (2004)
4 more