› 1p9y

X-ray diffraction
2.15Å resolution

Ribosome binding of E. coli Trigger Factor mutant F44L.

Released:
DOI: 10.2210/pdb1p9y/pdb
PDB entry 1p9y coloured by chain, front view.
PDB entry 1p9y coloured by chain, side view.
PDB entry 1p9y coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Chaperone binding at the ribosomal exit tunnel.
Kristensen O, Gajhede M
Structure 11 1547-56 (2003)
PMID: 14656439

Function and Biology Details

Reaction catalysed: 
Peptidylproline (omega=180) = peptidylproline (omega=0)
Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141658 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Trigger factor Chains: A, B
Molecule details ›
Chains: A, B
Length: 121 amino acids
Theoretical weight: 13.33 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A850 (Residues: 1-118; Coverage: 27%)
Gene names: JW0426, b0436, tig
Sequence domains: Bacterial trigger factor protein (TF)
Structure domains: Trigger factor ribosome-binding domain

Ligands and Environments

1 bound ligand:
Ligand ACY 3 x ACY
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX II BEAMLINE I711
Spacegroup: R32
Unit cell:
a: 71.901Å b: 71.901Å c: 257.659Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.2 0.2 0.247
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Breaking on through to the other side: protein export through the bacterial Sec system.
Chatzi et al. (2013)
3 more

1 mention without citation

The Structure of Treponema pallidum Tp0624 Reveals a Modular Assembly of Divergently Functionalized and Previously Uncharacterized Domains.
Parker et al. (2016)