› 1pdn

X-ray diffraction
2.5Å resolution

CRYSTAL STRUCTURE OF A PAIRED DOMAIN-DNA COMPLEX AT 2.5 ANGSTROMS RESOLUTION REVEALS STRUCTURAL BASIS FOR PAX DEVELOPMENTAL MUTATIONS

Released:
DOI: 10.2210/pdb1pdn/pdb
PDB entry 1pdn coloured by chain, front view.
PDB entry 1pdn coloured by chain, side view.
PDB entry 1pdn coloured by chain, top view.
Source organism: Drosophila melanogaster
Primary publication:
Crystal structure of a paired domain-DNA complex at 2.5 A resolution reveals structural basis for Pax developmental mutations.
Xu W, Rould MA, Jun S, Desplan C, Pabo CO
Cell 80 639-50 (1995)
PMID: 7867071

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-114370 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Segmentation protein paired Chain: C
Molecule details ›
Chain: C
Length: 128 amino acids
Theoretical weight: 14.2 KDa
Source organism: Drosophila melanogaster
Expression system: BL21(DE3)
UniProt:
  • Canonical: P06601 (Residues: 27-154; Coverage: 21%)
Gene names: CG6716, prd
Sequence domains: 'Paired box' domain
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain
DNA (5'-D(*AP*AP*CP*GP*TP*CP*AP*CP*GP*GP*TP*TP*GP*AP*C)-3') Chain: A
Molecule details ›
Chain: A
Length: 15 nucleotides
Theoretical weight: 4.59 KDa
Source organism: Drosophila melanogaster
Expression system: Not provided
DNA (5'-D(*TP*TP*GP*TP*CP*AP*AP*CP*CP*GP*TP*GP*AP*CP*G)-3') Chain: B
Molecule details ›
Chain: B
Length: 15 nucleotides
Theoretical weight: 4.58 KDa
Source organism: Drosophila melanogaster
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P212121
Unit cell:
a: 39.6Å b: 68.6Å c: 100.5Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.234 0.234 0.284
Expression system: Not provided

Quick links

Citations

35 review citations

Review and update of mutations causing Waardenburg syndrome.
Pingault et al. (2010)
34 more

12 mentions without citation

Classification of intrinsically disordered regions and proteins.
van der Lee et al. (2014)
11 more