› 1pdq

X-ray diffraction
1.76Å resolution

Polycomb chromodomain complexed with the histone H3 tail containing trimethyllysine 27.

Released:
DOI: 10.2210/pdb1pdq/pdb
PDB entry 1pdq coloured by chain, front view.
PDB entry 1pdq coloured by chain, side view.
PDB entry 1pdq coloured by chain, top view.
Source organisms:
Primary publication:
Molecular basis for the discrimination of repressive methyl-lysine marks in histone H3 by Polycomb and HP1 chromodomains.
Fischle W, Wang Y, Jacobs SA, Kim Y, Allis CD, Khorasanizadeh S
Genes Dev 17 1870-81 (2003)
PMID: 12897054

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150635 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Polycomb group protein Pc Chain: A
Molecule details ›
Chain: A
Length: 72 amino acids
Theoretical weight: 8.85 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli
UniProt:
  • Canonical: P26017 (Residues: 14-77; Coverage: 16%)
Gene names: CG7618, Pc
Sequence domains: Chromo (CHRromatin Organisation MOdifier) domain
Structure domains: OB fold (Dihydrolipoamide Acetyltransferase, E2P)
Histone H3.3 Chain: B
Molecule details ›
Chain: B
Length: 18 amino acids
Theoretical weight: 1.93 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P84243 (Residues: 16-33; Coverage: 13%)
Gene names: H3-3A, H3-3B, H3.3A, H3.3B, H3F3, H3F3A, H3F3B, PP781

Ligands and Environments

No bound ligands
1 modified residue:
Ligand M3L 1 x M3L

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: I212121
Unit cell:
a: 32.12Å b: 75.81Å c: 80.46Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.223 0.223 0.253
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

216 review citations

The mammalian epigenome.
Bernstein et al. (2007)
215 more

8 mentions without citation

Structure and mechanisms of lysine methylation recognition by the chromodomain in gene transcription.
Yap et al. (2011)
7 more