1pfb

X-ray diffraction
1.4Å resolution

Structural Basis for specific binding of polycomb chromodomain to histone H3 methylated at K27

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
hetero tetramer
PDBe Complex ID:
PDB-CPX-139005 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Polycomb group protein Pc Chain: A
Molecule details ›
Chain: A
Length: 55 amino acids
Theoretical weight: 6.81 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli
UniProt:
  • Canonical: P26017 (Residues: 23-77; Coverage: 14%)
Gene names: CG7618, Pc
Sequence domains: Chromo (CHRromatin Organisation MOdifier) domain
Structure domains: OB fold (Dihydrolipoamide Acetyltransferase, E2P)
Histone H3, embryonic Chain: B
Molecule details ›
Chain: B
Length: 11 amino acids
Theoretical weight: 1.16 KDa
Source organism: Strongylocentrotus purpuratus
Expression system: Not provided
UniProt:
  • Canonical: P06352 (Residues: 21-31; Coverage: 8%)

Ligands and Environments

3 bound ligands:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X26C
Spacegroup: I212121
Unit cell:
a: 32.429Å b: 77.033Å c: 77.273Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.197 0.197 0.216
Expression systems:
  • Escherichia coli
  • Not provided