› 1pff

X-ray diffraction
2.5Å resolution

Crystal Structure of Homocysteine alpha-, gamma-lyase at 1.8 Angstroms

Released:
DOI: 10.2210/pdb1pff/pdb
PDB entry 1pff coloured by chain, front view.
PDB entry 1pff coloured by chain, side view.
PDB entry 1pff coloured by chain, top view.
Source organism: Trichomonas vaginalis
Entry authors: Allen TW, Sridhar V, Prasad SG, Han Q, Xu M, Tan Y, Hoffman RM, Ramaswamy S

Function and Biology Details

Reaction catalysed: 
(1a) L-methionine = methanethiol + 2-aminobut-2-enoate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127537 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
L-methionine gamma-lyase Chains: A, B
Molecule details ›
Chains: A, B
Length: 331 amino acids
Theoretical weight: 35.93 KDa
Source organism: Trichomonas vaginalis
UniProt:
  • Canonical: O15565 (Residues: 68-398; Coverage: 83%)
Gene name: mgl2
Sequence domains: Cys/Met metabolism PLP-dependent enzyme
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand EDO 21 x EDO
Ligand PEG 3 x PEG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: C2
Unit cell:
a: 166.634Å b: 48.476Å c: 75.606Å
α: 90° β: 110.33° γ: 90°
R-values:
R R work R free
0.179 0.179 0.208

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