› 1pfo

X-ray diffraction
2.2Å resolution

PERFRINGOLYSIN O

Released:
DOI: 10.2210/pdb1pfo/pdb
PDB entry 1pfo coloured by chain, front view.
PDB entry 1pfo coloured by chain, side view.
PDB entry 1pfo coloured by chain, top view.
Source organism: Clostridium perfringens
Primary publication:
Structure of a cholesterol-binding, thiol-activated cytolysin and a model of its membrane form.
Rossjohn J, Feil SC, McKinstry WJ, Tweten RK, Parker MW
Cell 89 685-92 (1997)
PMID: 9182756

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142995 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Perfringolysin O Chain: A
Molecule details ›
Chain: A
Length: 500 amino acids
Theoretical weight: 55.83 KDa
Source organism: Clostridium perfringens
Expression system: Escherichia coli
UniProt:
  • Canonical: P0C2E9 (Residues: 1-500; Coverage: 100%)
Gene names: CPE0163, pfo, pfoA, pfoR
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-6A
Spacegroup: C2221
Unit cell:
a: 46.987Å b: 178.292Å c: 174.207Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.211 0.211 0.268
Expression system: Escherichia coli

Quick links

Citations

64 review citations

Listeria pathogenesis and molecular virulence determinants.
Vázquez-Boland et al. (2001)
63 more

49 mentions without citation

Pneumococcal virulence factors: structure and function.
Jedrzejas MJ. (2001)
48 more