› 1pfr

X-ray diffraction
2.2Å resolution

RIBONUCLEOSIDE-DIPHOSPHATE REDUCTASE 1 BETA CHAIN

Released:
DOI: 10.2210/pdb1pfr/pdb
PDB entry 1pfr coloured by chain, front view.
PDB entry 1pfr coloured by chain, side view.
PDB entry 1pfr coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Crystal structure of reduced protein R2 of ribonucleotide reductase: the structural basis for oxygen activation at a dinuclear iron site.
Logan DT, Su XD, Aberg A, Regnström K, Hajdu J, Eklund H, Nordlund P
Structure 4 1053-64 (1996)
PMID: 8805591

Function and Biology Details

Reaction catalysed: 
2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H(2)O = ribonucleoside diphosphate + thioredoxin
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159694 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribonucleoside-diphosphate reductase 1 subunit beta Chains: A, B
Molecule details ›
Chains: A, B
Length: 340 amino acids
Theoretical weight: 39.59 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P69924 (Residues: 2-341; Coverage: 90%)
Gene names: JW2229, b2235, ftsB, nrdB
Sequence domains: Ribonucleotide reductase, small chain
Structure domains: Ribonucleotide Reductase, subunit A

Ligands and Environments

2 bound ligands:
Ligand FE 4 x FE
Ligand HG 8 x HG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX9.5
Spacegroup: P212121
Unit cell:
a: 74.3Å b: 85.5Å c: 115.7Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.179 0.179 not available
Expression system: Escherichia coli

Quick links

Citations

14 review citations

Ribonucleotide reductases.
Jordan et al. (1998)
13 more

7 mentions without citation

Dioxygen Activation by Nonheme Diiron Enzymes: Diverse Dioxygen Adducts, High-Valent Intermediates, and Related Model Complexes.
Jasniewski et al. (2018)
6 more