› 1pgu

X-ray diffraction
2.3Å resolution

YEAST ACTIN INTERACTING PROTEIN 1 (AIP1), Se-Met PROTEIN, MONOCLINIC CRYSTAL FORM

Released:
DOI: 10.2210/pdb1pgu/pdb
PDB entry 1pgu coloured by chain, front view.
PDB entry 1pgu coloured by chain, side view.
PDB entry 1pgu coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
The structure of Aip1p, a WD repeat protein that regulates Cofilin-mediated actin depolymerization.
Voegtli WC, Madrona AY, Wilson DK
J Biol Chem 278 34373-9 (2003)
PMID: 12807914

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155507 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Actin-interacting protein 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 615 amino acids
Theoretical weight: 67.7 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P46680 (Residues: 1-615; Coverage: 100%)
Gene names: AIP1, YM9582.17C, YMR092C
Sequence domains: WD domain, G-beta repeat
Structure domains: YVTN repeat-like/Quinoprotein amine dehydrogenase

Ligands and Environments

1 bound ligand:
Ligand ZN 2 x ZN
1 modified residue:
Ligand MSE 12 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P21
Unit cell:
a: 60.8Å b: 154.53Å c: 69Å
α: 90° β: 90.65° γ: 90°
R-values:
R R work R free
0.205 0.202 0.257
Expression system: Escherichia coli

Quick links

Citations

9 review citations

Structure and function of WD40 domain proteins.
Xu et al. (2011)
8 more

6 mentions without citation

Mechanisms of actin disassembly and turnover.
Goode et al. (2023)
5 more