› 1pi6

X-ray diffraction
2.5Å resolution

YEAST ACTIN INTERACTING PROTEIN 1 (Aip1), ORTHORHOMBIC CRYSTAL FORM

Released:
DOI: 10.2210/pdb1pi6/pdb
PDB entry 1pi6 coloured by chain, front view.
PDB entry 1pi6 coloured by chain, side view.
PDB entry 1pi6 coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
The structure of Aip1p, a WD repeat protein that regulates Cofilin-mediated actin depolymerization.
Voegtli WC, Madrona AY, Wilson DK
J Biol Chem 278 34373-9 (2003)
PMID: 12807914

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-155506 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Actin-interacting protein 1 Chain: A
Molecule details ›
Chain: A
Length: 615 amino acids
Theoretical weight: 67.42 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P46680 (Residues: 1-615; Coverage: 100%)
Gene names: AIP1, YM9582.17C, YMR092C
Sequence domains: WD domain, G-beta repeat
Structure domains: YVTN repeat-like/Quinoprotein amine dehydrogenase

Ligands and Environments

1 bound ligand:
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-1
Spacegroup: P21212
Unit cell:
a: 69.12Å b: 154.43Å c: 62.11Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.222 0.219 0.269
Expression system: Escherichia coli

Quick links

Citations

9 review citations

Structure and function of WD40 domain proteins.
Xu et al. (2011)
8 more

3 mentions without citation

Aip1 and cofilin promote rapid turnover of yeast actin patches and cables: a coordinated mechanism for severing and capping filaments.
Okada et al. (2006)
2 more