› 1pie

X-ray diffraction
2.1Å resolution

Crystal Structure of Lactococcus lactis Galactokinase Complexed with Galactose

Released:
DOI: 10.2210/pdb1pie/pdb
PDB entry 1pie coloured by chain, front view.
PDB entry 1pie coloured by chain, side view.
PDB entry 1pie coloured by chain, top view.
Source organism: Lactococcus lactis
Primary publication:
Molecular structure of galactokinase.
Thoden JB, Holden HM
J Biol Chem 278 33305-11 (2003)
PMID: 12796487

Function and Biology Details

Reaction catalysed: 
ATP + alpha-D-galactose = ADP + alpha-D-galactose 1-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193116 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Galactokinase Chain: A
Molecule details ›
Chain: A
Length: 419 amino acids
Theoretical weight: 46.08 KDa
Source organism: Lactococcus lactis
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9R7D7 (Residues: 1-399; Coverage: 100%)
Gene names: L0028, LL1983, galK
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand GLA 1 x GLA
Ligand PO4 1 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: I23
Unit cell:
a: 143.4Å b: 143.4Å c: 143.4Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.204 0.199 0.266
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Structure and function of enzymes of the Leloir pathway for galactose metabolism.
Holden et al. (2003)
5 more

6 mentions without citation

Therapeutic potential of SH2 domain-containing inositol-5'-phosphatase 1 (SHIP1) and SHIP2 inhibition in cancer.
Fuhler et al. (2012)
5 more