› 1pj8

X-ray diffraction
2.2Å resolution

Structure of a ternary complex of proteinase K, mercury and a substrate-analogue hexapeptide at 2.2 A resolution

Released:
DOI: 10.2210/pdb1pj8/pdb
PDB entry 1pj8 coloured by chain, front view.
PDB entry 1pj8 coloured by chain, side view.
PDB entry 1pj8 coloured by chain, top view.
Source organism: Parengyodontium album
Primary publication:
Structure of a ternary complex of proteinase K, mercury, and a substrate-analogue hexa-peptide at 2.2 A resolution.
Saxena AK, Singh TP, Peters K, Fittkau S, Visanji M, Wilson KS, Betzel C
Proteins 25 195-201 (1996)
PMID: 8811735

Function and Biology Details

Reaction catalysed: 
Hydrolysis of keratin, and of other proteins with subtilisin-like specificity. Hydrolyzes peptide amides.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139281 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Proteinase K Chain: A
Molecule details ›
Chain: A
Length: 279 amino acids
Theoretical weight: 28.93 KDa
Source organism: Parengyodontium album
UniProt:
  • Canonical: P06873 (Residues: 106-384; Coverage: 76%)
Gene name: PROK
Sequence domains: Subtilase family
Structure domains: Peptidase S8/S53 domain
6-residue peptide (N-Ac-PAPFPA-NH2) Chain: I
Molecule details ›
Chain: I
Length: 7 amino acids
Theoretical weight: 597 Da
Source organism: Parengyodontium album
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand HG 2 x HG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: OTHER
Spacegroup: P43212
Unit cell:
a: 68.28Å b: 68.28Å c: 107.87Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.17 0.17 not available
Expression system: Not provided

Quick links

Citations

7 citation in other articles

Enhancement of enzyme activity through three-phase partitioning: crystal structure of a modified serine proteinase at 1.5 A resolution.
Singh et al. (2001)
6 more

2 mentions without citation

Therapeutic strategies for Covid-19 based on molecular docking and dynamic studies to the ACE-2 receptors, Furin, and viral spike proteins.
Khattab et al. (2022)
1 more