› 1pjs

X-ray diffraction
2.4Å resolution

The co-crystal structure of CysG, the multifunctional methyltransferase/dehydrogenase/ferrochelatase for siroheme synthesis, in complex with it NAD cofactor

Released:
DOI: 10.2210/pdb1pjs/pdb
PDB entry 1pjs coloured by chain, front view.
PDB entry 1pjs coloured by chain, side view.
PDB entry 1pjs coloured by chain, top view.
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium
Primary publication:
CysG structure reveals tetrapyrrole-binding features and novel regulation of siroheme biosynthesis.
Stroupe ME, Leech HK, Daniels DS, Warren MJ, Getzoff ED
Nat Struct Biol 10 1064-73 (2003)
PMID: 14595395

Function and Biology Details

Reactions catalysed: 
Precorrin-2 + NAD(+) = sirohydrochlorin + NADH
(1a) S-adenosyl-L-methionine + uroporphyrinogen III = S-adenosyl-L-homocysteine + precorrin-1
Siroheme + 2 H(+) = sirohydrochlorin + Fe(2+)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150613 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:

Ligands and Environments


Cofactor: Ligand SAH 2 x SAH

Cofactor: Ligand NAD 2 x NAD
2 bound ligands:
Ligand PGE 2 x PGE
Ligand PO4 1 x PO4
1 modified residue:
Ligand SEP 2 x SEP

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P212121
Unit cell:
a: 60.124Å b: 120.686Å c: 130.178Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.239 0.239 0.281
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Architectures, mechanisms and molecular evolution of natural product methyltransferases.
Liscombe et al. (2012)
5 more

5 mentions without citation

Diversity of the reaction mechanisms of SAM-dependent enzymes.
Sun et al. (2021)
4 more