› 1pju

X-ray diffraction
2.15Å resolution

Unbound form of Tomato Inhibitor-II

Released:
DOI: 10.2210/pdb1pju/pdb
PDB entry 1pju coloured by chain, front view.
PDB entry 1pju coloured by chain, side view.
PDB entry 1pju coloured by chain, top view.
Source organism: Solanum lycopersicum
Primary publication:
Unbound form of tomato inhibitor-II reveals interdomain flexibility and conformational variability in the reactive site loops.
Barrette-Ng IH, Ng KK, Cherney MM, Pearce G, Ghani U, Ryan CA, James MN
J Biol Chem 278 31391-400 (2003)
PMID: 12788916

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domain:
Structure domain:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-138519 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Wound-induced proteinase inhibitor 2 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 123 amino acids
Theoretical weight: 13.47 KDa
Source organism: Solanum lycopersicum
UniProt:
  • Canonical: P05119 (Residues: 26-148; Coverage: 100%)
Sequence domains: Potato type II proteinase inhibitor family
Structure domains: Wheat Germ Agglutinin (Isolectin 2); domain 1

Ligands and Environments

1 bound ligand:
Ligand SO4 8 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: C2
Unit cell:
a: 58.688Å b: 106.607Å c: 81.804Å
α: 90° β: 104.12° γ: 90°
R-values:
R R work R free
0.208 0.206 0.25

Quick links

Citations

2 review citations

Complementation of intramolecular interactions for structural-functional stability of plant serine proteinase inhibitors.
Joshi et al. (2013)
1 more

5 mentions without citation

Peptide-based protease inhibitors from plants.
Hellinger et al. (2019)
4 more