› 1pl5

X-ray diffraction
2.5Å resolution

Crystal Structure Analysis of the Sir4p C-terminal Coiled Coil

Released:
DOI: 10.2210/pdb1pl5/pdb
PDB entry 1pl5 coloured by chain, front view.
PDB entry 1pl5 coloured by chain, side view.
PDB entry 1pl5 coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
The Sir4 C-terminal coiled coil is required for telomeric and mating type silencing in Saccharomyces cerevisiae.
Murphy GA, Spedale EJ, Powell ST, Pillus L, Schultz SC, Chen L
J Mol Biol 334 769-80 (2003)
PMID: 14636601

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146020 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Regulatory protein SIR4 Chains: A, S
Molecule details ›
Chains: A, S
Length: 142 amino acids
Theoretical weight: 15.96 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P11978 (Residues: 1217-1358; Coverage: 11%)
Gene names: ASD1, SIR4, STE9, UTH2, YD9934.12, YDR227W
Structure domains: Single helix bin

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-C
Spacegroup: P65
Unit cell:
a: 108.884Å b: 108.884Å c: 75.342Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.261 0.261 0.288
Expression system: Escherichia coli

Quick links

Citations

7 review citations

The Nuts and Bolts of Transcriptionally Silent Chromatin in Saccharomyces cerevisiae.
Gartenberg et al. (2016)
6 more

3 mentions without citation

Structural attributes for the recognition of weak and anomalous regions in coiled-coils of myosins and other motor proteins.
Sunitha et al. (2012)
2 more