› 1pn4

X-ray diffraction
2.35Å resolution

Crystal structure of 2-enoyl-CoA hydratase 2 domain of Candida tropicalis multifunctional enzyme type 2 complexed with (3R)-hydroxydecanoyl-CoA.

Released:
DOI: 10.2210/pdb1pn4/pdb
PDB entry 1pn4 coloured by chain, front view.
PDB entry 1pn4 coloured by chain, side view.
PDB entry 1pn4 coloured by chain, top view.
Source organism: Candida tropicalis
Primary publication:
A two-domain structure of one subunit explains unique features of eukaryotic hydratase 2.
Koski MK, Haapalainen AM, Hiltunen JK, Glumoff T
J Biol Chem 279 24666-72 (2004)
PMID: 15051722

Function and Biology Details

Reactions catalysed: 
(R)-3-hydroxyacyl-CoA + NAD(+) = 3-oxoacyl-CoA + NADH
(3R)-3-hydroxyacyl-CoA = (2E)-2-enoyl-CoA + H(2)O
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149628 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Peroxisomal hydratase-dehydrogenase-epimerase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 280 amino acids
Theoretical weight: 31.4 KDa
Source organism: Candida tropicalis
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P22414 (Residues: 627-906; Coverage: 31%)
Sequence domains: MaoC like domain
Structure domains: Hotdog Thioesterase

Ligands and Environments

2 bound ligands:
Ligand HDC 3 x HDC
Ligand EDO 4 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X13
Spacegroup: P21
Unit cell:
a: 48.64Å b: 151.29Å c: 81.62Å
α: 90° β: 90.5° γ: 90°
R-values:
R R work R free
0.174 0.171 0.225
Expression system: Escherichia coli BL21

Quick links

Citations

7 review citations

Peroxisomal beta-oxidation--a metabolic pathway with multiple functions.
Poirier et al. (2006)
6 more

8 mentions without citation

Active site comparisons and catalytic mechanisms of the hot dog superfamily.
Labonte et al. (2013)
7 more