› 1po3

X-ray diffraction
3.4Å resolution

Crystal structure of ferric citrate transporter FecA in complex with ferric citrate

Released:
DOI: 10.2210/pdb1po3/pdb
PDB entry 1po3 coloured by chain, front view.
PDB entry 1po3 coloured by chain, side view.
PDB entry 1po3 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structural evidence for iron-free citrate and ferric citrate binding to the TonB-dependent outer membrane transporter FecA.
Yue WW, Grizot S, Buchanan SK
J Mol Biol 332 353-68 (2003)
PMID: 12948487

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146442 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fe(3+) dicitrate transport protein FecA Chains: A, B
Molecule details ›
Chains: A, B
Length: 751 amino acids
Theoretical weight: 83.17 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P13036 (Residues: 34-774; Coverage: 100%)
Gene names: JW4251, b4291, fecA
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand FLC 4 x FLC
Ligand FE 4 x FE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X9B
Spacegroup: P21
Unit cell:
a: 85.489Å b: 147.003Å c: 96.131Å
α: 90° β: 110.58° γ: 90°
R-values:
R R work R free
0.283 0.239 0.314
Expression system: Escherichia coli BL21

Quick links

Citations

27 review citations

Siderophore-based iron acquisition and pathogen control.
Miethke et al. (2007)
26 more

6 mentions without citation

TonB-dependent transporters: regulation, structure, and function.
Noinaj et al. (2010)
5 more