› 1po5

X-ray diffraction
1.6Å resolution

Structure of mammalian cytochrome P450 2B4

Released:
DOI: 10.2210/pdb1po5/pdb
PDB entry 1po5 coloured by chain, front view.
PDB entry 1po5 coloured by chain, side view.
PDB entry 1po5 coloured by chain, top view.
Source organism: Oryctolagus cuniculus
Primary publication:
An open conformation of mammalian cytochrome P450 2B4 at 1.6-A resolution.
Scott EE, He YA, Wester MR, White MA, Chin CC, Halpert JR, Johnson EF, Stout CD
Proc Natl Acad Sci U S A 100 13196-201 (2003)
PMID: 14563924

Function and Biology Details

Reaction catalysed: 
RH + [reduced NADPH--hemoprotein reductase] + O(2) = ROH + [oxidized NADPH--hemoprotein reductase] + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-132309 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cytochrome P450 2B4 Chain: A
Molecule details ›
Chain: A
Length: 476 amino acids
Theoretical weight: 54.14 KDa
Source organism: Oryctolagus cuniculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P00178 (Residues: 20-491; Coverage: 96%)
Gene name: CYP2B4
Sequence domains: Cytochrome P450
Structure domains: Cytochrome P450

Ligands and Environments


Cofactor: Ligand HEM 1 x HEM
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: C2221
Unit cell:
a: 57.09Å b: 114.2Å c: 133.95Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.217 not available 0.289
Expression system: Escherichia coli

Quick links

Citations

38 review citations

Heme enzyme structure and function.
Poulos TL. (2014)
37 more

33 mentions without citation

Structural features of cytochromes P450 and ligands that affect drug metabolism as revealed by X-ray crystallography and NMR.
Gay et al. (2010)
32 more