1pp8

X-ray diffraction
3.05Å resolution

crystal structure of the T. vaginalis IBP39 Initiator binding domain (IBD) bound to the alpha-SCS Inr element

Released:
Source organism: Trichomonas vaginalis

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assemblies composition:
hetero trimer (preferred)
monomeric
PDBe Complex ID:
PDB-CPX-114400 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
39 kDa initiator binding protein Chains: F, M, O, P, U, V
Molecule details ›
Chains: F, M, O, P, U, V
Length: 132 amino acids
Theoretical weight: 15.34 KDa
Source organism: Trichomonas vaginalis
Expression system: Escherichia coli
UniProt:
  • Canonical: Q95VR4 (Residues: 1-126; Coverage: 37%)
Gene name: IBP39
Sequence domains: Transcription-initiator DNA-binding domain IBD
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain
ALPHA-SCS INR Chains: E, I, K, Y
Molecule details ›
Chains: E, I, K, Y
Length: 12 nucleotides
Theoretical weight: 3.6 KDa
Source organism: Trichomonas vaginalis
Expression system: Not provided
ALPHA-SCS INR Chains: G, J, R, T
Molecule details ›
Chains: G, J, R, T
Length: 12 nucleotides
Theoretical weight: 3.73 KDa
Source organism: Trichomonas vaginalis
Expression system: Not provided

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: F23
Unit cell:
a: 292Å b: 292Å c: 292Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.272 0.269 0.311
Expression systems:
  • Escherichia coli
  • Not provided