› 1ppa

X-ray diffraction
2Å resolution

THE CRYSTAL STRUCTURE OF A LYSINE 49 PHOSPHOLIPASE A2 FROM THE VENOM OF THE COTTONMOUTH SNAKE AT 2.0 ANGSTROMS RESOLUTION

Released:
DOI: 10.2210/pdb1ppa/pdb
PDB entry 1ppa coloured by chain, front view.
PDB entry 1ppa coloured by chain, side view.
PDB entry 1ppa coloured by chain, top view.
Source organism: Agkistrodon piscivorus piscivorus
Primary publication:
The crystal structure of a lysine 49 phospholipase A2 from the venom of the cottonmouth snake at 2.0-A resolution.
Holland DR, Clancy LL, Muchmore SW, Ryde TJ, Einspahr HM, Finzel BC, Heinrikson RL, Watenpaugh KD
J Biol Chem 265 17649-56 (1990)
PMID: 2120215

Function and Biology Details

Reaction catalysed: 
Phosphatidylcholine + H(2)O = 1-acylglycerophosphocholine + a carboxylate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-137605 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Basic phospholipase A2 homolog AppP2 Chain: A
Molecule details ›
Chain: A
Length: 121 amino acids
Theoretical weight: 13.99 KDa
Source organism: Agkistrodon piscivorus piscivorus
Expression system: Not provided
UniProt:
  • Canonical: P04361 (Residues: 1-121; Coverage: 100%)
Sequence domains: Phospholipase A2
Structure domains: Phospholipase A2 domain

Ligands and Environments

1 bound ligand:
Ligand ANL 1 x ANL
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P41212
Unit cell:
a: 71.53Å b: 71.53Å c: 57.59Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.157 0.157 not available
Expression system: Not provided

Quick links

Citations

6 review citations

An overview of lysine-49 phospholipase A2 myotoxins from crotalid snake venoms and their structural determinants of myotoxic action.
Lomonte et al. (2003)
5 more

5 mentions without citation

Carboxylic ester hydrolases: Classification and database derived from their primary, secondary, and tertiary structures.
Chen et al. (2016)
4 more