› 1pry

X-ray diffraction
1.97Å resolution

Structure Determination of Fibrillarin Homologue From Hyperthermophilic Archaeon Pyrococcus furiosus (Pfu-65527)

Released:
DOI: 10.2210/pdb1pry/pdb
PDB entry 1pry coloured by chain, front view.
PDB entry 1pry coloured by chain, side view.
PDB entry 1pry coloured by chain, top view.
Source organism: Pyrococcus furiosus
Primary publication:
Structure determination of fibrillarin from the hyperthermophilic archaeon Pyrococcus furiosus.
Deng L, Starostina NG, Liu ZJ, Rose JP, Terns RM, Terns MP, Wang BC
Biochem Biophys Res Commun 315 726-32 (2004)
PMID: 14975761

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186499 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase Chain: A
Molecule details ›
Chain: A
Length: 227 amino acids
Theoretical weight: 25.78 KDa
Source organism: Pyrococcus furiosus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q8U4M2 (Residues: 1-227; Coverage: 100%)
Gene names: PF0059, flpA
Sequence domains: Fibrillarin
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: P212121
Unit cell:
a: 43.967Å b: 62.082Å c: 103.363Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.21 0.204 0.259
Expression system: Escherichia coli BL21

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Citations

7 review citations

Non-coding RNAs: lessons from the small nuclear and small nucleolar RNAs.
Matera et al. (2007)
6 more