1pue

X-ray diffraction
2.1Å resolution

PU.1 ETS DOMAIN-DNA COMPLEX

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-114405 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Transcription factor PU.1 Chains: E, F
Molecule details ›
Chains: E, F
Length: 89 amino acids
Theoretical weight: 10.61 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P17433 (Residues: 171-259; Coverage: 33%)
Gene names: Sfpi-1, Sfpi1, Spi1
Sequence domains: Ets-domain
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain
DNA (5'-D(*AP*AP*AP*AP*AP*GP*GP*GP*GP*AP*AP*GP*TP*GP*GP*G)-3') Chains: A, C
Molecule details ›
Chains: A, C
Length: 16 nucleotides
Theoretical weight: 5.09 KDa
Source organism: synthetic construct
Expression system: Not provided
DNA (5'-D(*TP*CP*CP*CP*AP*CP*TP*TP*CP*CP*CP*CP*TP*TP*TP*T)-3') Chains: B, D
Molecule details ›
Chains: B, D
Length: 16 nucleotides
Theoretical weight: 4.71 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: LURE BEAMLINE D41A, null
Spacegroup: C2
Unit cell:
a: 89.1Å b: 101.9Å c: 55.6Å
α: 90° β: 111.2° γ: 90°
R-values:
R R work R free
0.225 0.225 0.287
Expression systems:
  • Escherichia coli
  • Not provided