› 1puu

X-ray diffraction
2.3Å resolution

Mistletoe lectin I in complex with lactose

Released:
DOI: 10.2210/pdb1puu/pdb
PDB entry 1puu coloured by chain, front view.
PDB entry 1puu coloured by chain, side view.
PDB entry 1puu coloured by chain, top view.
Source organism: Viscum album
Primary publication:
Mistletoe lectin I in complex with galactose and lactose reveals distinct sugar-binding properties.
Mikeska R, Wacker R, Arni R, Singh TP, Mikhailov A, Gabdoulkhakov A, Voelter W, Betzel C
Acta Crystallogr Sect F Struct Biol Cryst Commun 61 17-25 (2005)
PMID: 16508080

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of the N-glycosidic bond at one specific adenosine on the 28S rRNA.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160520 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (5 distinct):
Beta-galactoside-specific lectin 1 chain A isoform 1 Chain: A
Molecule details ›
Chain: A
Length: 249 amino acids
Theoretical weight: 27.23 KDa
Source organism: Viscum album
UniProt:
  • Canonical: P81446 (Residues: 34-282; Coverage: 47%)
Sequence domains: Ribosome inactivating protein
Structure domains:
Beta-galactoside-specific lectin 1 chain B Chain: B
Molecule details ›
Chain: B
Length: 263 amino acids
Theoretical weight: 28.53 KDa
Source organism: Viscum album
UniProt:
  • Canonical: P81446 (Residues: 302-564; Coverage: 50%)
Sequence domains: Ricin-type beta-trefoil lectin domain
Structure domains: Trefoil (Acidic Fibroblast Growth Factor, subunit A)

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, FUL
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: BGC, GAL
Carbohydrate polymer
5 bound ligands:
Ligand NAG 2 x NAG
Ligand GOL 16 x GOL
Ligand CL 3 x CL
Ligand DIO 1 x DIO
Ligand SO4 3 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X11
Spacegroup: P6522
Unit cell:
a: 106.278Å b: 106.278Å c: 312.329Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.25 0.207 0.232

Quick links

Citations

11 citation in other articles

AB-type lectin (toxin/agglutinin) from mistletoe: differences in affinity of the two galactoside-binding Trp/Tyr-sites and regulation of their functionality by monomer/dimer equilibrium.
Jiménez et al. (2006)
10 more

1 mention without citation

Mistletoe lectin I in complex with galactose and lactose reveals distinct sugar-binding properties.
Mikeska et al. (2005)