› 1pv1

X-ray diffraction
2.3Å resolution

Crystal Structure Analysis of Yeast Hypothetical Protein: YJG8_YEAST

Released:
DOI: 10.2210/pdb1pv1/pdb
PDB entry 1pv1 coloured by chain, front view.
PDB entry 1pv1 coloured by chain, side view.
PDB entry 1pv1 coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Structural characterization and reversal of the natural organophosphate resistance of a D-type esterase, Saccharomyces cerevisiae S-formylglutathione hydrolase.
Legler PM, Kumaran D, Swaminathan S, Studier FW, Millard CB
Biochemistry 47 9592-601 (2008)
PMID: 18707125

Function and Biology Details

Reaction catalysed: 
S-formylglutathione + H(2)O = glutathione + formate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154102 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
S-formylglutathione hydrolase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 299 amino acids
Theoretical weight: 33.98 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P40363 (Residues: 1-299; Coverage: 100%)
Gene names: HRE299, J1102, YJL068C
Sequence domains: Putative esterase
Structure domains: alpha/beta hydrolase

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12C
Spacegroup: P212121
Unit cell:
a: 121.366Å b: 66.929Å c: 171.949Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.255 0.255 0.298
Expression system: Escherichia coli

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Citations

2 review citations

Chemical approaches to detect and analyze protein sulfenic acids.
Furdui et al. (2014)
1 more

10 mentions without citation

Thioesterases: a new perspective based on their primary and tertiary structures.
Cantu et al. (2010)
9 more