› 1pvg

X-ray diffraction
1.8Å resolution

Crystal Structure of the ATPase region of Saccharomyces Cerevisiae topoisomerase II

Released:
DOI: 10.2210/pdb1pvg/pdb
PDB entry 1pvg coloured by chain, front view.
PDB entry 1pvg coloured by chain, side view.
PDB entry 1pvg coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Structure of the topoisomerase II ATPase region and its mechanism of inhibition by the chemotherapeutic agent ICRF-187.
Classen S, Olland S, Berger JM
Proc Natl Acad Sci U S A 100 10629-34 (2003)
PMID: 12963818

Function and Biology Details

Reaction catalysed: 
ATP-dependent breakage, passage and rejoining of double-stranded DNA
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA topoisomerase 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 418 amino acids
Theoretical weight: 48.57 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P06786 (Residues: 1-413; Coverage: 29%)
Gene names: N2244, TOP2, TOR3, YNL088W
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand MG 2 x MG
Ligand ANP 2 x ANP
1 modified residue:
Ligand MSE 20 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P212121
Unit cell:
a: 58.456Å b: 71.081Å c: 216.132Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.209 0.206 0.241
Expression system: Escherichia coli

Quick links

Citations

29 review citations

Targeting DNA topoisomerase II in cancer chemotherapy.
Nitiss JL. (2009)
28 more

8 mentions without citation

Analysis of the eukaryotic topoisomerase II DNA gate: a single-molecule FRET and structural perspective.
Collins et al. (2009)
7 more