› 1pwf

X-ray diffraction
1.16Å resolution

One Sugar Pucker Fits All: Pairing Versatility Despite Conformational Uniformity in TNA

Released:
DOI: 10.2210/pdb1pwf/pdb
PDB entry 1pwf coloured by chain, front view.
PDB entry 1pwf coloured by chain, side view.
PDB entry 1pwf coloured by chain, top view.
Source organism: Synthetic construct
Primary publication:
Why does TNA cross-pair more strongly with RNA than with DNA? an answer from X-ray analysis.
Pallan PS, Wilds CJ, Wawrzak Z, Krishnamurthy R, Eschenmoser A, Egli M
Angew Chem Int Ed Engl 42 5893-5 (2003)
PMID: 14673929

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-114414 (preferred)
Entry contents:
1 distinct DNA molecule
Macromolecule:
5'-D(*GP*CP*GP*TP*AP*(2MU)P*(FA2)P*CP*GP*C)-3' Chains: A, B
Molecule details ›
Chains: A, B
Length: 10 nucleotides
Theoretical weight: 3.06 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand SPM 1 x SPM
2 modified residues:
Ligand 2MU 2 x 2MU
Ligand FA2 2 x FA2

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 5ID-B
Spacegroup: P212121
Unit cell:
a: 24.429Å b: 44.522Å c: 47.332Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.133 0.133 0.188
Expression system: Not provided

Quick links

Citations

11 review citations

Etiology of potentially primordial biomolecular structures: from vitamin B12 to the nucleic acids and an inquiry into the chemistry of life's origin: a retrospective.
Eschenmoser A. (2011)
10 more