› 1px5

X-ray diffraction
1.74Å resolution

Crystal structure of the 2'-specific and double-stranded RNA-activated interferon-induced antiviral protein 2'-5'-oligoadenylate synthetase

Released:
DOI: 10.2210/pdb1px5/pdb
PDB entry 1px5 coloured by chain, front view.
PDB entry 1px5 coloured by chain, side view.
PDB entry 1px5 coloured by chain, top view.
Source organism: Sus scrofa
Primary publication:
Crystal structure of the 2'-specific and double-stranded RNA-activated interferon-induced antiviral protein 2'-5'-oligoadenylate synthetase.
Hartmann R, Justesen J, Sarkar SN, Sen GC, Yee VC
Mol Cell 12 1173-85 (2003)
PMID: 14636576

Function and Biology Details

Reaction catalysed: 
3 ATP = pppA2'p5'A2'p5'A + 2 diphosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-173356 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
2'-5'-oligoadenylate synthase 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 349 amino acids
Theoretical weight: 40.42 KDa
Source organism: Sus scrofa
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q29599 (Residues: 1-349; Coverage: 100%)
Gene name: OAS1
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand SO4 4 x SO4
1 modified residue:
Ligand YCM 4 x YCM

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.3
Spacegroup: P21
Unit cell:
a: 46.524Å b: 132.601Å c: 57.305Å
α: 90° β: 99.19° γ: 90°
R-values:
R R work R free
0.187 0.187 0.224
Expression system: Escherichia coli BL21(DE3)

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Citations

24 review citations

Interferon-inducible antiviral effectors.
Sadler et al. (2008)
23 more

18 mentions without citation

RNA-specific ribonucleotidyl transferases.
Martin et al. (2007)
17 more