› 1pxw

X-ray diffraction
1.94Å resolution

Crystal structure of L7Ae sRNP core protein from Pyrococcus abyssii

Released:
DOI: 10.2210/pdb1pxw/pdb
PDB entry 1pxw coloured by chain, front view.
PDB entry 1pxw coloured by chain, side view.
PDB entry 1pxw coloured by chain, top view.
Source organism: Pyrococcus abyssi
Primary publication:
Purification, crystallization and preliminary X-ray diffraction data of L7Ae sRNP core protein from Pyrococcus abyssii.
Charron C, Manival X, Charpentier B, Branlant C, Aubry A
Acta Crystallogr D Biol Crystallogr 60 122-4 (2004)
PMID: 14684904

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158535 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Large ribosomal subunit protein eL8 Chains: A, B
Molecule details ›
Chains: A, B
Length: 128 amino acids
Theoretical weight: 14.08 KDa
Source organism: Pyrococcus abyssi
Expression system: Escherichia coli
UniProt:
  • Canonical: P62008 (Residues: 1-123; Coverage: 100%)
Gene names: PAB0460, PYRAB06750, rpl7ae
Sequence domains: Ribosomal protein L7Ae/L30e/S12e/Gadd45 family
Structure domains: 60s Ribosomal Protein L30; Chain: A;

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P21212
Unit cell:
a: 67.19Å b: 112.55Å c: 34.51Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.229 0.227 0.278
Expression system: Escherichia coli

Quick links

Citations

5 citation in other articles

Reconstitution of archaeal H/ACA small ribonucleoprotein complexes active in pseudouridylation.
Charpentier et al. (2005)
4 more

2 mentions without citation

Structure of H/ACA RNP protein Nhp2p reveals cis/trans isomerization of a conserved proline at the RNA and Nop10 binding interface.
Koo et al. (2011)
1 more