› 1pz4

X-ray diffraction
1.35Å resolution

The structural determination of an insect (mosquito) Sterol Carrier Protein-2 with a ligand bound C16 Fatty Acid at 1.35 A resolution

Released:
DOI: 10.2210/pdb1pz4/pdb
PDB entry 1pz4 coloured by chain, front view.
PDB entry 1pz4 coloured by chain, side view.
PDB entry 1pz4 coloured by chain, top view.
Source organism: Aedes aegypti
Primary publication:
The structural determination of an insect sterol carrier protein-2 with a ligand-bound C16 fatty acid at 1.35-A resolution.
Dyer DH, Lovell S, Thoden JB, Holden HM, Rayment I, Lan Q
J Biol Chem 278 39085-91 (2003)
PMID: 12855689

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-183139 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
SCP2 domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 116 amino acids
Theoretical weight: 12.86 KDa
Source organism: Aedes aegypti
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q86PR3 (Residues: 1-110; Coverage: 100%)
Gene names: 110681488, SCP-2
Sequence domains: SCP-2 sterol transfer family
Structure domains: SCP2 sterol-binding domain

Ligands and Environments

1 bound ligand:
Ligand PLM 1 x PLM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P212121
Unit cell:
a: 29.3Å b: 46.1Å c: 70.4Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.187 0.187 0.226
Expression system: Escherichia coli BL21

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Citations

4 review citations

Intracellular sterol dynamics.
Mesmin et al. (2009)
3 more

24 mentions without citation

3(10) helices in channels and other membrane proteins.
Vieira-Pires et al. (2010)
23 more