› 1pzf

X-ray diffraction
2.2Å resolution

T.gondii LDH1 ternary complex with APAD+ and oxalate

Released:
DOI: 10.2210/pdb1pzf/pdb
PDB entry 1pzf coloured by chain, front view.
PDB entry 1pzf coloured by chain, side view.
PDB entry 1pzf coloured by chain, top view.
Source organism: Toxoplasma gondii
Primary publication:
Structure of Toxoplasma gondii LDH1: active-site differences from human lactate dehydrogenases and the structural basis for efficient APAD+ use.
Kavanagh KL, Elling RA, Wilson DK
Biochemistry 43 879-89 (2004)
PMID: 14744130

Function and Biology Details

Reaction catalysed: 
(S)-lactate + NAD(+) = pyruvate + NADH
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161038 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
L-lactate dehydrogenase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 331 amino acids
Theoretical weight: 35.74 KDa
Source organism: Toxoplasma gondii
Expression system: Escherichia coli
UniProt:
  • Canonical: P90613 (Residues: 1-329; Coverage: 100%)
Gene name: TGRH88_076470
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand A3D 4 x A3D
1 bound ligand:
Ligand OXL 4 x OXL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P21
Unit cell:
a: 68.065Å b: 124.976Å c: 86.686Å
α: 90° β: 106.09° γ: 90°
R-values:
R R work R free
0.178 0.178 0.218
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Potential biomarkers and their applications for rapid and reliable detection of malaria.
Jain et al. (2014)
1 more

1 mention without citation

An atomic-resolution view of neofunctionalization in the evolution of apicomplexan lactate dehydrogenases.
Boucher et al. (2014)