› 1pzi

X-ray diffraction
1.99Å resolution

Heat-Labile Enterotoxin B-Pentamer Complexed With Nitrophenyl Galactoside 2a

Released:
DOI: 10.2210/pdb1pzi/pdb
PDB entry 1pzi coloured by chain, front view.
PDB entry 1pzi coloured by chain, side view.
PDB entry 1pzi coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
3,5-Substituted phenyl galactosides as leads in designing effective cholera toxin antagonists; synthesis and crystallographic studies.
Mitchell DD, Pickens JC, Korotkov K, Fan E, Hol WG
Bioorg Med Chem 12 907-20 (2004)
PMID: 14980603

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152492 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Heat-labile enterotoxin B chain Chains: D, E, F, G, H
Molecule details ›
Chains: D, E, F, G, H
Length: 103 amino acids
Theoretical weight: 11.81 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P32890 (Residues: 22-124; Coverage: 100%)
Gene names: eltB, ltpB
Sequence domains: Heat-labile enterotoxin beta chain
Structure domains: OB fold (Dihydrolipoamide Acetyltransferase, E2P)

Ligands and Environments

1 bound ligand:
Ligand 1DM 5 x 1DM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21
Unit cell:
a: 60.817Å b: 78.683Å c: 62.3Å
α: 90° β: 116.2° γ: 90°
R-values:
R R work R free
0.158 0.154 0.221
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Structural comparison of lactose permease and the glycerol-3-phosphate antiporter: members of the major facilitator superfamily.
Abramson et al. (2004)
1 more

3 mentions without citation

Lipid-mediated endocytosis.
Ewers et al. (2011)
2 more