› 1pzj

X-ray diffraction
1.46Å resolution

Cholera Toxin B-Pentamer Complexed With Nitrophenyl Galactoside 5

Released:
DOI: 10.2210/pdb1pzj/pdb
PDB entry 1pzj coloured by chain, front view.
PDB entry 1pzj coloured by chain, side view.
PDB entry 1pzj coloured by chain, top view.
Source organism: Vibrio cholerae
Primary publication:
3,5-Substituted phenyl galactosides as leads in designing effective cholera toxin antagonists; synthesis and crystallographic studies.
Mitchell DD, Pickens JC, Korotkov K, Fan E, Hol WG
Bioorg Med Chem 12 907-20 (2004)
PMID: 14980603

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176410 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cholera enterotoxin B-subunit Chains: D, E, F, G, H
Molecule details ›
Chains: D, E, F, G, H
Length: 103 amino acids
Theoretical weight: 11.62 KDa
Source organism: Vibrio cholerae
Expression system: Escherichia coli
UniProt:
  • Canonical: Q57193 (Residues: 22-124; Coverage: 100%)
Gene names: ERS013165_03981, ctxB
Sequence domains: Heat-labile enterotoxin beta chain
Structure domains: OB fold (Dihydrolipoamide Acetyltransferase, E2P)

Ligands and Environments

2 bound ligands:
Ligand 15B 3 x 15B
Ligand J15 2 x J15
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: C2
Unit cell:
a: 101.754Å b: 65.998Å c: 77.362Å
α: 90° β: 105.44° γ: 90°
R-values:
R R work R free
0.117 0.115 0.159
Expression system: Escherichia coli

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Citations

2 review citations

Structural comparison of lactose permease and the glycerol-3-phosphate antiporter: members of the major facilitator superfamily.
Abramson et al. (2004)
1 more