› 1pzr

Solution NMR

Structure of fused docking domains from the erythromycin polyketide synthase (DEBS), a model for the interaction between DEBS2 and DEBS3: the B domain

Released:
DOI: 10.2210/pdb1pzr/pdb
PDB entry 1pzr coloured by chain, ensemble of 7 models, front view.
PDB entry 1pzr coloured by chain, ensemble of 7 models, side view.
PDB entry 1pzr coloured by chain, ensemble of 7 models, top view.
Source organism: Saccharopolyspora erythraea
Primary publication:
The structure of docking domains in modular polyketide synthases.
Broadhurst RW, Nietlispach D, Wheatcroft MP, Leadlay PF, Weissman KJ
Chem Biol 10 723-31 (2003)
PMID: 12954331

Function and Biology Details

Reaction catalysed: 
Propanoyl-CoA + 6 (2S)-methylmalonyl-CoA + 6 NADPH = 6-deoxyerythronolide B + 7 CoA + 6 CO(2) + H(2)O + 6 NADP(+)
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169840 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
6-deoxyerythronolide-B synthase EryA2, modules 3 and 4 Chains: A, B
Molecule details ›
Chains: A, B
Length: 60 amino acids
Theoretical weight: 6.84 KDa
Source organism: Saccharopolyspora erythraea
Expression system: Escherichia coli
UniProt:
  • Canonical: Q03132 (Residues: 3548-3567; Coverage: 1%)
Gene name: eryA
Structure domains: Histone, subunit A

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: simulated annealing
Expression system: Escherichia coli

Quick links

Citations

38 review citations

Combinatorial biosynthesis of reduced polyketides.
Weissman et al. (2005)
37 more

6 mentions without citation

Protein-protein interactions in "cis-AT" polyketide synthases.
Dodge et al. (2018)
5 more