› 1q0s

X-ray diffraction
2.3Å resolution

Binary Structure of T4DAM with AdoHcy

Released:
DOI: 10.2210/pdb1q0s/pdb
PDB entry 1q0s coloured by chain, front view.
PDB entry 1q0s coloured by chain, side view.
PDB entry 1q0s coloured by chain, top view.
Source organism: Escherichia virus T4
Primary publication:
Structure of the bacteriophage T4 DNA adenine methyltransferase.
Yang Z, Horton JR, Zhou L, Zhang XJ, Dong A, Zhang X, Schlagman SL, Kossykh V, Hattman S, Cheng X
Nat Struct Biol 10 849-55 (2003)
PMID: 12937411

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine + adenine in DNA = S-adenosyl-L-homocysteine + N-6-methyladenine in DNA
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-137620 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA adenine methylase Chain: A
Molecule details ›
Chain: A
Length: 259 amino acids
Theoretical weight: 30.46 KDa
Source organism: Escherichia virus T4
Expression system: Escherichia coli
UniProt:
  • Canonical: P04392 (Residues: 1-259; Coverage: 100%)
Gene name: DAM
Sequence domains: D12 class N6 adenine-specific DNA methyltransferase
Structure domains:

Ligands and Environments


Cofactor: Ligand SAH 1 x SAH
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X26C
Spacegroup: P212121
Unit cell:
a: 58.6Å b: 63.1Å c: 72.7Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.222 0.222 0.266
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Dam methylation: coordinating cellular processes.
Løbner-Olesen et al. (2005)
3 more

4 mentions without citation

Transition from nonspecific to specific DNA interactions along the substrate-recognition pathway of dam methyltransferase.
Horton et al. (2005)
3 more