› 1q14

X-ray diffraction
2.5Å resolution

Structure and autoregulation of the yeast Hst2 homolog of Sir2

Released:
DOI: 10.2210/pdb1q14/pdb
PDB entry 1q14 coloured by chain, front view.
PDB entry 1q14 coloured by chain, side view.
PDB entry 1q14 coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Structure and autoregulation of the yeast Hst2 homolog of Sir2.
Zhao K, Chai X, Clements A, Marmorstein R
Nat Struct Biol 10 864-71 (2003)
PMID: 14502267

Function and Biology Details

Reaction catalysed: 
(1a) [protein]-N(6)-acetyl-L-lysine + NAD(+) = [protein]-N(6)-(1,1-(5-adenosylyl-alpha-D-ribose-1,2-di-O-yl)ethyl)-L-lysine + nicotinamide
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156860 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NAD-dependent protein deacetylase HST2 Chain: A
Molecule details ›
Chain: A
Length: 361 amino acids
Theoretical weight: 40.38 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P53686 (Residues: 1-357; Coverage: 100%)
Gene names: HST2, LPA2C, YPL015C
Sequence domains: Sir2 family
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand ZN 1 x ZN
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: R32
Unit cell:
a: 103.554Å b: 103.554Å c: 177.346Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.239 0.219 0.251
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

13 review citations

The biochemistry of sirtuins.
Sauve et al. (2006)
12 more

11 mentions without citation

Sirtuins: Sir2-related NAD-dependent protein deacetylases.
North et al. (2004)
10 more