› 1q1j

X-ray diffraction
2.5Å resolution

Crystal Structure Analysis of anti-HIV-1 Fab 447-52D in complex with V3 peptide

Released:
DOI: 10.2210/pdb1q1j/pdb
PDB entry 1q1j coloured by chain, front view.
PDB entry 1q1j coloured by chain, side view.
PDB entry 1q1j coloured by chain, top view.
Source organisms:
Primary publication:
Structural rationale for the broad neutralization of HIV-1 by human monoclonal antibody 447-52D.
Stanfield RL, Gorny MK, Williams C, Zolla-Pazner S, Wilson IA
Structure 12 193-204 (2004)
PMID: 14962380

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-226721 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Fab 447-52D, light chain Chains: L, M
Molecule details ›
Chains: L, M
Length: 215 amino acids
Theoretical weight: 22.68 KDa
Source organism: Homo sapiens
Structure domains: Immunoglobulins
Fab 447-52D, heavy chain Chains: H, I
Molecule details ›
Chains: H, I
Length: 231 amino acids
Theoretical weight: 24.76 KDa
Source organism: Homo sapiens
Structure domains: Immunoglobulins
Surface protein gp120 Chains: P, Q
Molecule details ›
Chains: P, Q
Length: 16 amino acids
Theoretical weight: 1.83 KDa
Source organism: HIV-1 M:B_MN
Expression system: Not provided
UniProt:
  • Canonical: P05877 (Residues: 310-323; Coverage: 2%)
Gene name: env

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: P21
Unit cell:
a: 76.673Å b: 74.903Å c: 100.047Å
α: 90° β: 100.57° γ: 90°
R-values:
R R work R free
0.25 0.25 0.285
Expression system: Not provided

Quick links

Citations

16 review citations

GP120: target for neutralizing HIV-1 antibodies.
Pantophlet et al. (2006)
15 more

18 mentions without citation

Max Bergmann lecture protein epitope mimetics in the age of structural vaccinology.
Robinson JA. (2013)
17 more