› 1q1l

X-ray diffraction
2.05Å resolution

Crystal Structure of Chorismate Synthase

Released:
DOI: 10.2210/pdb1q1l/pdb
PDB entry 1q1l coloured by chain, front view.
PDB entry 1q1l coloured by chain, side view.
PDB entry 1q1l coloured by chain, top view.
Source organism: Aquifex aeolicus
Primary publication:
Crystal structure of chorismate synthase from Aquifex aeolicus reveals a novel beta alpha beta sandwich topology.
Viola CM, Saridakis V, Christendat D
Proteins 54 166-9 (2004)
PMID: 14705034

Function and Biology Details

Reaction catalysed: 
5-O-(1-carboxyvinyl)-3-phosphoshikimate = chorismate + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130316 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Chorismate synthase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 401 amino acids
Theoretical weight: 44.93 KDa
Source organism: Aquifex aeolicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O66493 (Residues: 1-398; Coverage: 100%)
Gene names: aq_081, aroC
Sequence domains: Chorismate synthase
Structure domains: Chorismate synthase AroC

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 28 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-ID-B
Spacegroup: C2
Unit cell:
a: 153.428Å b: 95.959Å c: 113.462Å
α: 90° β: 93.04° γ: 90°
R-values:
R R work R free
0.209 0.209 0.246
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

The shikimate pathway: review of amino acid sequence, function and three-dimensional structures of the enzymes.
Mir et al. (2015)
5 other articles

2 mentions without citation

The Blueprint of a Minimal Cell: MiniBacillus.
Reuß et al. (2016)
1 more